ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION

EFFECTOFl-NAPHTHYLACETAMIDE(NAAM)ONGROWTHOF

CARROTCELLCULTURETRANSFORMED

W ITHAGROBACTERIUM

TUMEFACIENS

K.Z.Gamburg, L .V .Gamanets, T.A.Markova andO .M .Barykova

Siberian Institute o fPlantPhysiologyandBiochemistry, SiberianBranch o ftheRussian

Academy o fSciences, 664033, Irkutsk, P.O.Box 1243, RUSSIA

Growth o f carrot cell culture transformed within

A.t.

8628 was inhibited

by 25 and 50 mg/l NAAm. Adapted cell lines (ACL) were obtained from the

initial culture (IC) after several subcultures in the medium with 25-50 mg/l

NAAm which can grow in the presence of NAAm at the same rate as IC in

the NAAm absence. ACL had greater activity of amidohydrolase on a fresh

wheight base than IC due to greater protein content in soluble enzyme

extracts. Growth of ACL in medium without NAAm exceeded significantly

the growth of IC in the same medium. The difference in growth and the

resistance to NAAm of ACL disappeared gradually during 3 successive

subcultures in the NAAm absence. NAAm is assumed to induce temporal

modification o f carrot cells which allows them to maintain growth rate in the

NAAm presence at the same level as the growth rate of IC in the medium

without NAAm. This modification is not likely to be associated with a decrease

of the expression of gene 2 of Ti-plasmide coding amidohydrolase.

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