ЭлБиб

PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION

USEOFGENETICTRANSFORMATIONFORINCREASEOFPOTATO

RESISTANCETOPATHOGENS

V.A.Avetisov, Yu.V.Davydova, A.P.Sharova, *T.E.Mishina, A.A.Shadenkov,

L.A.Serdobinsky, G.G.Krivtsov, B.F.Vanjushin, **G.N.Cherepneva,

S.Melik-Saridsov

A ll Russian Institute o fagricultural biotechnology, 127550Moscow, Thimiriasevskaja

st. 42; *Moscowagricultural academy o fa name K.A. Thimiriasev, 127550Moscow,

Thimiriasevskaja st. 49;

**Thimiriazev Institute o fPlant Physiology RAS, 127276Moscow,

Botanichescaja st.35.

The alteration o f the hormonal balance in plant can strongly change

many genes expression, including genes, connected with action of protective

reactions. Therefore the interesting approach to increase of plant resistance

to pathogens is reception o f transgenic plants with changed phytohormonal

status. Potato transgenic plants with additional isopenteniltransferase gene

received, using strain pGV2206

Agrobacterium tumefaciens,

in which genes

o f auxins synthesis were deleted. Transgenic regenerants with the increased

endogenous cytokinins contents had also increased o f activity level of plant

ch itin a s e s , p la y ing by a im p o rta n t role in p lan t p ro te c tion from

phytopathogenic fungi. Besides, they distinguish oneself by increased

resistance to one o f major factors o f pathogenesis - microorganisms

pectinases. Thus, received data, enabling to assume, that endogenous

cytokinins can participate in protective reactions o f plants by means o f

increase o f plant chitinases activity and increase o f plant resistance to

pathogens pectinases. Other approach was connected with the introduction

in potato genom o f isolated from radish seeds gene o f peptide antibiotics,

protecting plants from pathogens. The analysis received regenerants is

carried out.

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