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T O T IP O T E N C Y A N D M O R P H O G E N E S IS OF P L A N T C E L L S

IN V IT R O

AGEMORPHOGENESISSPESIFICI TYOFEGGPLANT (SOLANUM

MELONGENAL.) STRUCTURES

IN VITRO

A.G.Yusufov, Z.M.Alieva

Dagestan State University, Makhachkala, 367025, Gadzhieva-street, 4ЗА.

There studied the morphogenesis specificity of explants (E) o f

hypocotyls, leaves and cotyledons (EH, EL and EC)

in vitro

in eggplant

(Solanum melongena L.) plants (variety Almaz) at the age of 7-10 (I) and 25-

30 (II) days on the Murashige-Skoog medium with the addition of IAA:6-

benzylaminopurine in different combination (mg./litre).

O f all structures formed actively callus. However respectively their age

there was increase of there lethality and decrease of their growth. 80% of

EC formed roots in IAA:6-benzylaminopurine variant (3:1), other combinations

- 30-40%. Variant II EC formed roots only with addition of growth regulators

(2:2,1:3) in isolated cases. EH formed vigorous callus, though its size

decreased a little in II. EH callus roots formed only in variant I on the minimum

medium. With this EH polarization increased by shoot formation on the

upper end and root - on callus ofthe lowerone. When IAA:6-benzylaminopurine

(0.5:2.5) EH developed many shoots on the upper end, on the low e rone -

vigorous callus without roots. With such correlation of regulators in variant II

only callus on the lower end formed.

Development of EL callus was accompanied with root formation within

20-30 % (different variants). EL roots formed mainly when weak callus

development. In separate cases (10-30%) on EL callus a bud formation took

place, better- in variant IAA:6-benzylaminopurine (1:3). When correlation of

growth regulators (2:1) EL root formation within 60-70% was observed even

on adult plant.

In variants o f combinations o f salt stress (NaCI, 1%) and growth

regulators (0.5:2.5) root development of all explants in variants I and II wasn't

watched, though callus development took place. When salt stress high

lethality o f EH was observed. Good survival of EC was accompanied only

with weak callus development.

Dispersion analysis of age and salt stress influence estimation showed

for explants respectively 15% and 30%.

Structure and age differentiation influences morphogenesis specifity

vitro

of eggplant explants. Its concrete epigenetic nature needs clearing -

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