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T O T IP O T E N C Y A N D M O R P H O G E N E S IS OF P L A N T C E L L S IN V IT R O
CALLUS ANDMORPHOGENESISINTHECULTUREOFWILDCEREALS
R.K.Salyaev, V.M.Sumtsova, S.V.Lankevich
Siberian Institute o fPlantPhysiology andBiochemistiy Siberian Branch ofthe Russian
Academy o fSciences P.O.Box 1243, Irkutsk 664033 Fax:(3952)310754,
E-Mail:
The ability for callus- and morphogenesis was examined in six wild
cereals widespread in the Irkutsk region and Pribaikalie: Agropyron cristatum
L., Brachypodium pinnatum L., Elymus mutabilis L., Elymus sibiricus L.,
Elytrigia repens L. and Festuca pratensis L. in order to create a most
complete cell culture collection of cereals living under severe climatic
conditions of the East-Siberian region to include their genomes in constructing
new wheat forms by combining recent methods of cellular and genetic
engineering with traditional breeding. Mature seeds were used as explants
and the modified MS medium as the nutrient solution.
A rather high ability for callus formation (55% to 90%) was observed in
the above species. Elymus sibiricus L. and Agropyron cristatum L. were
found to possess the highest morphogenic potential. The yield of callus was
70-90% of the total amount of the explants. The number of the morphogenic
zones formed amounted to 20%. A wave-like character of appearance and
disappearance o f morphogenic zones was observed during prolonged period
of cultivation.
Kinetin was found to affect the formation of morphogenic zones and
regeneration in Agropyron cristatum L.
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