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T O T IP O T E N C Y AN D M O R P H O G E N E S IS OF P L A N T C E L L S

IN V IT R O

SHOOTREGENERATION INCENTAURIUMERYTHRAEANGILIB.ROOT

CULTURE

Angelina Subotic, SnezanaBudimir, DragoljubGrubisic

Institutefo r Biological Research, 29 November 142,11000 Belgrade, Yugoslavia E - a il:

Centaurium erythraea G ilib., Centaury, is a medicinal plant of the

Gentianaceae family. This is a biannual plant with leaves arranged in a rosette.

Like some other species of Gentianaceae family, Centaury contains specific

compounds used as a drug.

Adventitious

roots of

Centaurium erythraea

derived from

in vitro

grown seedlings were cultured on half-strength MS

medium supplemented with various auxins and cytokinins. Cultures were

kept both in light and in darkness. Within four weeks of culture adventitious

buds were developed directly on explants without an intervening callus tissue.

Significant differences in frequency o f shoot formation were found among

the tested cytokinins and auxins. Shoot production was achieved at high

frequency on explants cultured on the medium supplemented with CPPU,

KlN and BAP at 1-3 mM . The lower average number o f shoots was obtained

in the presence of 2iP and diphenylurea. A ll tested auxins showed inhibitory

effect on shoot formation. Light had a stimulatory effect on bud induction

and development in the presence of both cytokinins and auxins.

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