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TO T IPO T EN CY AND M O R PH O G E N E SIS OF PLANT C E L L S
IN V IT R O
THEPECULIARITIESOFLIGNIFICATIONOFCELLWALLSOF
BUCKWHEATCALLIWITHDIFFERENTMORPHOGENICABILITY
N.L.Rumyantseva,A.I.Valieva, N.A.Samokhvalova. A.R.Muklutov, M.V.Ageeva,
V.V.Lozovaya
Insitute ofBiology ofRussianAcademy ofSciences, Kazan,
E-Mail:Rumyantseva@sci.
kcn.ruWe looked for variation in lignin accumulation in cell walls of morphogenic
(MC) and non-morphogenic (NC) calli of tatar buckwheat (F.
tataricum
(L.)
Gaertn.) caused by changes in various culture conditions. Histological,
cytogenetic and physiological traits of these cultures have been studied. It
was shown, that lignin is localized in cell walls of MC and NC in different
ways. In NC, consisting of parenchyma ceils, it was deposited uniformly in
middle lamella, when in MC lignin was accumulated in cell corners of
proembryogenic cell complexes and in cell walls and middle lamella in cells
of soft tissue. When both calli were cultured on medium with BAP and upon
the light, the lignification of cell walls occurred, however, the increase of
lignification could not accompanied by vessel formation. Simultaneously
with lignin analysis we measured the activity of various forms of peroxidase
(soluble and bound with cell walls). The correlation between lignin
accumulation and the increase of peroxidase activity was found only in NC
cultured on the medium with BAP.
We suggested that the alterations in lignification of callus cell walls
both in course of culture cycle and under influence of various culture
conditions could be explained by two processes: by the increase of
lignification of cell walls themselves and by the alteration of the ratio of
various cell types (lignified and non-lignified), of which calli consisted in
normal state.
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