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T O T IPO T EN CY AND M O R PH O G EN E SIS OF PLANT C E L L S

IN V IT R O

INVESTIGATIONMORPHOGENESISANDEMBRYOGENESISOFSOME

CLOVER(

TRIFOLIUM PRATENSEL .)

VARIETIES

L.V.Mesnyankina,L.A.Solodkaja, V.V.Mazin*

WilliamsAll-Russia Research Institute ofFodder Crops, Moscow region, Lobnya

Scientific village

*All-Russia Research Institute ofAgriculturalBiotechnology, Moscow, Timiryazeva 42

The capacity o f callusogenesis, morphogenesis and embryogenesis

has been investigated for four prospective varieties of clover: VIC-7, Mars,

Rannij-2 and Arlington. The hypocotyl, cotyledon's leaf and leaf-stalk explants

were excised from plants grown aseptically. We used Gamborg B5, MS and

LS media with some modifications. Callus cultures were obtained from all

used varieties. All received callus cultures had morphogenic capacity and

preserved this property during subculturing.

LS medium with picloram (0,06 mg/l) and BA (0,1 mg/l) has been used

for morphogenesis. Varieties VIC-7, Rannij-2 have maximal capacity of

morphogenesis.

LS medium with 2,4-D (0,01 mg/l) and adenine (2,0 mg/l) has been used

for embryoids formation. For embryoids development the LS medium with

picloram (0,02 g/l) and BA (0,2 mg/l) was used. Regenerants have been

obtained from embryoids. NAA (0,2 mg/l) was used for root formation.

Ten regenerants of varieties Rannij-2 have been used for callus formation.

Callus cultures were obtained from all regenerants. Fertile seeds have

been obtained from some regenerants of variety Rannij-2.

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