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Аграрная наука Евро-Северо-Востока, №6(61), 2017 г.
государственной регистрации базы данных. База данных
аутосомных ДНК-маркеров свиней. №2015621623 (РФ) от
02.11.2015. Патентообладатель: Донской государственный
аграрный университет. Заявка №2015621146 09.09.2015.
Опубл. 20.11.2015, бюлл. №11(109).
3. Колосов A.Ю., Гетманцева Л.В., Леонова М.А.
Свидетельство о государственной регистрации компьютерной
программы. Информационная система учета SNP-генотипов
свиней по генам-маркерам продуктивности. №2015661461
(РФ)28.10.2015Патентообладатель:Донскойгосударственный
аграрный университет. Заявка №2015618229 от 09.09.2015.
Опубл. 20.11.2015, бюлл. №11(109).
4. Колосов A.Ю., Гетманцева Л.В., Леонова М.А.,
Бакоев С.Ю., Бакоев Н.Ф. Свидетельство о государственной
регистрации компьютерной программы. BLUP-model.
№2016661288 (РФ) 05.10.2016. Патентообладатель: Донской
государственный аграрный университет. Заявка№2016618783
12.08.2016. Опубл. 20.11.2016, бюлл. №11(121).
5. Kim G.W., Yoo J.Y., Kim H.Y. Association of geno-
type of POU1F1 intron 1 with carcass characteristics in cross-
bred pigs. J. Anim Sci Technol 2014; 56: 25.
6. Klimenko A., Usatov A., Getmantseva L., Kolosov
Yu., Tretyakova O., Bakoev S., Kostjunina O. and Zinovieva
N. Effect of melanocortin-4 receptor gene on growth and meat
traits in pigs raised in Russia. American Journal of Agricultur-
al and Biological Sciences, 2014, 9(2): 232-237.
7. Park S.-J., Ha J., Kim I.-S., Kwon S.G., Hwang
J.H., Park D.H., Kang D.G., Kim T.W., Kim S.W., Kim C.W.,
2015. Effects of LEP, GYS1, MYOD1, and MYF5 polymor-
phisms on pig economic traits. J. Ann Anim Sci., 15: 1-12.
8. Omelka R., Bauerová M., Mlynek J., Buchová B.,
Peškovičová D., Bulla J.. Effect of the estrogen receptor (ESR)
gene on reproductive traits of Large White, White Meaty and
Landrace pigs. Czech J. Anim. Sci., 50, 2005 (6): 249-253.
9. Piórkowska K., Ropka-Molik K., Oczkowicz M.,
Różycki M., Żukowski K. Association study of PIT1 and
GHRH SNPs with economically important traits in pigs of three
breeds reared in Poland. Anim Sci Pap Rep 2013; 31: 303-314.
10. Ropka-Molik K., Variability of mRNA abundance
of leukemia inhibitory factor gene (LIF) in porcine ovary,
oviduct and uterus tissues. Molecular Biology Reports 39(8):
7965-7972, 2012.
Creation of a panel of genetic markers for breeding of a large white breed pigs
on the reproductive fitness
Kolosov ., PhD in agriculture, associated professor,
Leonova M.A., PhD in agriculture, senior researcher,
Getmantseva L.V.., PhD in agriculture , head of laboratory
Donskoy state agrarian university, s. Persianovsky, Rostov region, Russia
The article presents studies of the effect of SNP of genes PRLR, LEP, ESR, FSHb, MC4R, LIF, POU1F1, GH on the repro-
ductive performance of large white pigs. The analysis was carried out using linear models of mixed type. For each variant of SNP,
a separate model was used; as a fixed factor in model the genotype for the gene in question was. Another fixed factor in all models
was the order of number of farrowing. A randomized factor was individual sow genotypes. As a result of the analysis a significant
superiority of the LEP/CC genotype over the LEP/TT genotype was established for all indicators of reproductive qualities: by the
number of pigs at birth on 1.55 h. (p = 0.02), for a piglets born alive on 1.8 h. (p = 0.009), by litter weight at birth on 1.91 kg (p =
0.05). According to the ESR gene animals with the ESR/BB genotype also outperformed all their analogues with the ESR/AA geno-
type (by the number of pigs at birth on 1.36 h. (p = 0.14), by piglets born alive on 0.93 h. (p = 0.005), by the litter weight at birth on
2.24 kg (p = 0.15) The LIF gene was used to determine the genotype LIF /BB (the number of piglets at birth + 1.35 h. (p = 0 , 15),
piglets born alive + 1.9 h. (p = 0.04), litter weight at birth + 2.24 kg (p = 0.24) relative to the LIF/AA genotype.) The superiority
of the PRLR/BB genotype was established for the PRLR gene (number of piglets at birth + 0.42 head (p = 0.4), piglets born alive
+ 0.57 h. (p = 0.25), litter weight at birth + 1.96 kg (p = 0.01) relative to the PRLR/AB genotype.) For other genes there are no
significant differences in the reproductive qualities between the genotypes. Based on the obtained results a panel of genetic markers
for evaluating reproductive qualities of large white pigs was developed, which includes the LEP, ESR, LIF , and PRLR genes.
Key words: SNP panel, pigs, markers, PRLR, LEP, ESR, FSHb, MC4R, LIF, POU1F1, GH, linear models
References
1. Samorè A.B., Fontanesi L. Genomic selection in
pigs: state of the art and perspectives. Italian Journal of An-
imal Science, 2016, 15(2). pp. 211-232.
2. Getmantseva L.V., Kolosov , Leonova M.A.,
Bakoev , Bakoev N.F., Radyuk A.V. Svidetel’stvo o
gosudarstvennoy registratsii bazy dannykh. Baza dannykh
autosomnykh DNK-markerov sviney. [Certificate of state
registration of the database. Database of autosomal DNA
markers of pigs]. Patent RF, no. 2015621623, 2015.
3. Kolosov ., Getmantseva L.V., Leonova M.A.
Svidetel’stvo o gosudarstvennoy registratsii komp’yuternoy
programmy. Informatsionnaya sistema ucheta SNP-genotipov
sviney po genam-markeram produktivnosti. [Certificate of
state registration of the computer program. Informational
system of SNP-genotypes accounting for pigs by genes-
markers of productivity]. Patent RF, no. 2015661461, 2015.
4. Kolosov , Getmantseva L.V., Leonova M.A.,
Bakoev ., Bakoev N.F. Svidetel’stvo o gosudarstvennoy
registratsii komp’yuternoy programmy. BLUP-model.
[Certificate of state registration of the computer program.
BLUP-model]. Patent RF, no. 2016661288, 2016.
5. Kim G.W., Yoo J.Y., Kim H.Y. Association of geno-
type of POU1F1 intron 1 with carcass characteristics in cross-
bred pigs. J. Anim Sci Technol, 2014. no. 56. p. 25
6. Klimenko A., Usatov A., Getmantseva L., Kolosov
Yu., Tretyakova O., Bakoev S., Kostjunina O. and Zinovieva
N. Effect of melanocortin-4 receptor gene on growth and meat
traits in pigs raised in Russia. American Journal of Agricultur-
al and Biological Sciences, 2014. no. 9(2). pp. 232-237.
7. Park S.-J., Ha J., Kim I.-S., Kwon S.G., Hwang
J.H., Park D.H., Kang D.G., Kim T.W., Kim S.W., Kim C.W.
Effects of LEP, GYS1, MYOD1, and MYF5 polymorphisms
on pig economic traits. J. Ann Anim Sci., 2015. no. 15.
pp. 1-12.
8. Omelka R., Bauerová M., Mlynek J., Buchová B.,
Peškovičová D., Bulla J. Effect of the estrogen receptor (ESR)
gene on reproductive traits of Large White, White Meaty and
Landrace pigs. Czech J. Anim. Sci., 2005. no. 50. pp. 249–253.
9. Piórkowska K., Ropka-Molik K., Oczkowicz M.,
Różycki M., Żukowski K. Association study of PIT1 and
GHRH SNPs with economically important traits in pigs of
three breeds reared in Poland. Anim Sci Pap Rep, 2013. no.
31. pp. 303-314.
10. Ropka-Molik K., Variability of mRNA abundance
of leukemia inhibitory factor gene (LIF) in porcine ovary, ovi-
duct and uterus tissues. Molecular Biology Reports 2012. no.
39(8). pp. 7965-7972.
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