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T a b. t.
Selection scheme for end-use quality breeding of winter
wheat at STUPICE.
Traits
P FI F2
Generation
F3 F4 F5 F6-n
Sedimentation (SDS) + + + + + +
Grain evaluation + + •+ - + + +
Protein content + + + + +
Falling number + + + + . +
Gluten content + + + + 4* + +
Gluten index 4* + + +
Glutenin composition -f + + + +
Mixograph + +
Alveograph + +
Baking quality + +
HMW glutenin composition is used for pre-selection in
early breeding generations (F3, F4). A higher total amount of
analyses is advisable. For classical electrophoresis from one
grain is necessary to analyse at least 5 grains per progeny. To
increase the labour capacity in the breeding process we use
mixed samples of 20-100 grains for electrophoretic analyses.
To verify this method we analysed artificially mixed samples
of two wheat varieties with different glutenin subunits and
bread making quality. The five percent share of cultivar can
be detected in a mixed sample on PAGE electrophoreograms
of HMW subunits of glutenins. The colour intensity of protein
bands enabled to detect the encreasing percentage of cultivar
with different HMW subunits in mixtures. The codominant
heritability enabled to detect parental or recombinant gametes,
homozygous or segregating progenies in HMW glutenin
subunits in F3-F5 plant progenies. In the breeding process in
later generations we analyse only selected lines which are not
homozygous in HMW glutenins. (Kubanek, Hanisova, Horcicka -
1999).
Mixograph is an efficient, simple and fast tool in wheat
breeding programme. In our programme we used mixograph
analyses since 1993 year. 35g, lOg, 5g and 2g versions were
developed. We are using lOg version. Different mixogram
parameters or indexes were used for evaluations (Martinant
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