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the confidence interval was: FL - 7.4%, L-10.8%. The correlation

coeficients between F

2

and derived F5f F

6

generations were

positive with values for different groups: FL-r=0.154 to 0.815” ,

L-r = 0.170-0.724*. Selection advance was for FL method

between 18.3-47.6% and heritability 0.41-0.71 depending on

the collection concerned. The required level of frost resistance

in Czech Republic is lower than in Russia. (Hanisova. Horcicka.

Hanis, 2000).

Doubled haploids by in vitro androgenesis have been

produced in cooperation with Academy of Sciences and Research

Institute of Plant Production in Prague-Ruzyne since 1992 in

Fp F

3

and F

4

generations. Our results has shown that performance

the DH lines are not so good as lines produced by conventional

systems. Till this days we have not positive results.

We expect in near future the introduction of molecular

technics in our breeding programme, mainly molecular markers

for quality and disease resistance.

Literature

Hanis M., Hanisova A. (1983):Estimatc of the yield potential of winter wheat cross

combinations from grain yield in the FI generation. Genet, a Slccht. *19: 213-220

Hanis M., Hanisova A., Havlicck Z. (1976): intcrplot competition and yield estimate

of four wheat cultivars in „Sccdmatic 6“ microplot trials. Cereal Res. Commun.,4:335-346.

Hanisova A., Horcicka P. Kubanck J (2000).: Mixograph test in wheat breeding

programme. Czech J.Gcnct.Plant.Brccd.,36: 34-44

Hanisova A., Horcicka P., Hanis M (2000): Screening on frost resistance in winter

wheat breeding. Abstracts of 6th International Wheat Conference 5-9 June 2000,

Budapest: 95

Hanisova A., Knytl V., Hanis M , Horcicka P., Klicova J. (1997), Breeding for

resistance to mildew and rusts in wheat, (in Protection of Cereals Crops against Harmful

Organisms, Kromcriz) 119-124.

Hanisova A., Petr J., Hruskova M. (1995): Possibility of evaluation breadmaking

quality for wheat breeding by Panasonic Automatic Bread Maker. Genet, a Slccht.,31:

277-284

Horcicka P., Markova O., Hanisova A. Effects of Scptoria and Pyrcnophora on

reduction of winter wheat yield (in press)

Johansson E., Svcnsson G. (1999) : Relationships among bread-making quality

parameters in Swedish wheats. J. Genet. Breed.,53: 93-98

Kubanck J., Hanisova A., Horcicka P.( 1999): The use of mixed samples for analyses

of HMW glutcnin subunits in wheat breeding. Czech J. Genet.Plant Breed.,35: 11-16

Martinant J.P., Nicolas Y., Bougucnncc A., Popincau Y., Saulnicr L., Branlard G.

(1998): Relationships between mixograph parameters and indices of wheat grain quality.

J. Cereal Sci.,27: 179-189

264

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