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PLANT GENETIC TRANSFORMATION, CELL SELECTION AND SOMATIC HYBRIDIZATION
GROWTHANDFORMING PHOTOSYNTHETICAPPARATUS IN
VALERIANA OWICINALISL. ROOTS, TRANSFORMEDW ITH
AGROBACTERIUMRHIZOGENES
R.A.Karnachnk, A.V.Guseva, I.N.Kniovkma*.
Tomsk State University, 634050, Tomsk, 36 Lenina S t., FAX: (3822) 222466, E-
M ail:olga @f
*InstitutetePhysiology o fPlants RAS, 127276, Moscow, 3 5 Botanicheskaya st.
The influence o f light ( 1 2 h/day; 6,1 V t/m2 ) on growth, sizes of
chloroplasts and pigments formation was investigated' in hairy root cultures
o f garden heliotrope, Control was in the dark. Light essentialy delaied the
growth o f the hairy roots. A fte r transfer on fresh medium the growth was
began through 10- 12 days, while in the dark this process was observered
through 2-3 days. Growth index reached 6,4 in the light and 35,2 in the dark.
IAA and abscisic asids activity was higher in the dark. Ratio free IAA / free
abscisic asid reached 1,74 in the light and 3,05 in the dark. Dark-grown
cultures were pale brown as opposed to slighly greenish in colour fo r the
light-grown cultures. The number of chloroplasts in cell of transformed roots
was sim ilar to that of palisade plant leaf cell. However, chloroplasts o f leaf
cells was larger 2-fold. We was found difference in volume o f cell, according
to one chloroplast, which reached 846,93 mkm3 in the roots and 417,88
mkm3 in the leaves. In the light was formated chlorophill ( [Chi a = 4,99 x 10
-3 mg/g dry weight, [Chi b] = 2,98 x 10 -3 mg/g d ry w e ig h t). In the dark
chlorophills wasn't found. The carotenoid concentration in the light was higher
2,3-fold than in the dark. Ratios chlorophill a/ b and chlorophill (a+b)/
carotenoids reached 1,7.
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