ЭлБиб

S O M A C L O N A L V A R IA TIO N S OF P L A N T S A N D C U L T U R E D C E L L S

USEOFSOMACLONALANDGAMETOCLONALVARIABILITYIN

PRODUCTIONOFNEWALLOPLASMICEUPLOIDANDANEUPLOID

WHEATLINESDERIVEDFROM

HORDEUML .x TRITICUML.

HYBRIDS

L.A.Pershina, O.M .Numerova, L.I.Belova, E.P.Devyatkina

Institute o fCytology and Genetics SiberianDepartment o fthe RussianAcademy o f

Sciences, 630090Novosibirsk, LavrentievAve, 10 E-Mail:

Different combinations o f barley-wheat hybrids were produced by using

methods of hormonal treatment of pollinated flowers and embryo culture.

However, fertility restoration remains a unresolved problem with most produced

hybrids. Here, we present the results o f selection o f somaclones induced

from the callus cultures o f young inflorescences o f the

H.vulgare

L. x

T.aestivum L.

and

H.geniculatum

All. x

T.aestivum L.

F1 hybrids. The

somaclones were selected for their seed-set ability after self-pollination and

backcrossing with wheat. The composition o f the induction (B-5) and

regeneration (B-5 and MS) media was changed by adding phytohormones

and colchicine in different combinations from one experiment to another. It

was found that the efficiency of somaclone formation with the development

o f viable gametes is related to both the composition of culture medium and

hybrid genotype. The backcross and self-pollinated progenies o f hybrids

w ith restored male fertility were involved in further production of androgenic

plants. This provided the accelerated production of recombinant homozygous

lines. Most gametoclones showed variation in plant height, spike length,

length o f the vegetative period. Thus, new alloplasmic, alloplasmic substitution

and additional lines of common wheat with barley cytoplasm were produced.

The lines appear promising in future studies.

The present work was supported by the Russian Foundation fo r

Fundamental Research (96-04-49973).

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