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TO T IPO T EN CY AND M O R PH O G EN E SIS OF PLANT C E L L S IN V IT R O
MORPHOGENESIS IN TISSUE CULTURE OFABELMOSCKUS
ESCVLENTUS L .
E.G.Holmuratov, G.B.Nasirova, A.G.Hamdam, S.A.Djataev
Institute o fGenetics andExperimentalBiology o fPlantsASRUz, P.0, 97, Tashkent 700
000, Uzbekistan, E-Mail: UZ
In spite o f "okra" (Abelmosckus esculentus L.) is widely spread in
some countries as food and technical culture, economical exploitation of it
is hindered because of low crop capacity of cultivated varieties. In this
connection the e labo ration o f regeneration m ethods and genetic
transformation of this plant is actual. Callus tissue was induced from 7-10
day sterile okra sprouts of the variety Clemson spineless. Medium for the
induction of callus tissue contained salts according to MS, vitamins B5
according to Gamborg, 3% glucose, phytohormones - 2,4-D, NAA, Kinetin,
BAP in different concentrations and combinations. Medium for the cultivation
of callus tissue besides above mentioned components contained such growth
stimulators as adenine, N6,N6-dimethyladenine (DAD), p-aminobenzoicacid
(PABA). Depending on induction conditions and ones of further cultivation,
the formation of embiyogenic zoneswhich gave the beginning for the formation
of somatic embryoids, roots and shoots were observed in callus tissue. In
some cases when callus tissues were induced on the medium containing
2,4-D and BAP and then were transferred to the medium with NAA and
secondly transferred to the medium with DAD, intensive root formation and
normally formed shoots were observed.
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