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TO T IPO T EN C Y AND M O R PH O G EN E SIS O F PLANT C E L L S

IN V IT R O

ORGANO-AND EMBRYOGENESIS INNARROW-LEAVED OLEASTER'S

ANTHERCULTURE

V.A.Halmanskaya

M.A.Lisavenko Research. Institute oj'Horticulturefo r Siberia, Barnaul, Russia

Conditions for haploids obtaining at cultivation of anthers of narrow-

leaved oleaster (Elaeagnus angustifolia L.) have been studied.

Anthers, containing mononucleate microspores at the early and middle

stage of development, were cultivated on different media. It was noted that

androgenesis induction only after callus formation took place. After callus

tissue obtaining two further morphogenetic directions may be observed:

organogenesis and embryogenesis.

Callus formation was noted on the all media used, but the best results

were observed on MS medium with 6-BAP, IBA and 2,4-D added.

Callus replantation on the medium for plant regeneration (MS with 6-

BAP 0,4-0,8 mg/l) promoted the meristem zones formation and a large

quantity of microbuds initiation. Some of microbuds were developed into

normal sprouts.

Formation of numerous embryoids along with the microbuds initiation

was observed. Experiments show that it would be betterto use 6-BAP as a

cytokinin active substance, but IBA and 2,4-D as auxin active ones fo rthe

embryogenetic structures development. The total amount of hormones should

not exceed 0,5 mg/l. The embryoids obtained were able to transform into

normal regenerants with a well-developed root system after moving them on

the hormoneless medium or on the medium with minimum hormones content

(up to 0,1 m g /l).

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