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TO T IPO T EN CY AND M O R PH O G E N E SIS O F PLANT C E L L S
IN V IT R O
UNUSUALTUMOR INDUCING FACTORANDPLANTREGENERATION IN
POTATO
N.D.Tikhenko
StateAgricultural University, Sankt-Petersburg, Russia
Eight genotypes (six commercially important Russian cultivars, dihaploid
(DH-30) and diploid
Solatium nigrum)
were selected for study of efficiency in
producing callus and regenerating plants. Among these genotypes 3 showed
significant differences fo r callus in-duction, and 5 different fo r plant
regeneration.
A fter extended culture period on the plants cv. Simvol was displayed
the new phenomenon. This unusual phenotype looks like a local tumours on
the leafs and stems. During in testing the effect of auxins on callus production
from plants with unusual phenotype was shown reducing callus growth but
in the same time explants have produced normal roots very active, which
suggested the tumour inducing factor changed the hormone balance in plants.
This tumor phenotype may be the result either of mutation, infection or
epigenetic changes. After repeated subculturing on regeneration medium,
shoots were induced by both leaf and stem pieces from plants with tumour
inducing factor. Plant regeneration ability o f those plants was very close to
parental plants. The study of mechanism o f this phenotypic change shows,
that this factor has the endogenic nature because it was maintained after
plant regeneration. The tumour frequency may be higher when plants are
cultivated with temperature above 25 °C and high humidity.
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