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TO T IPO T EN C Y AND M O R PH O G E N E SIS O F PLANT C E L L S IN V IT R O
INFLUENCEOFPHYTOHORMONESTO THEMORPHOGENIC
PROCESSES IN STACHYS TISSUE CULTURE
N.V.Khadeeva
N.I. Vavilov Institute o fGeneral Genetics RAS, Moscow,Gubkin str.3,117809, GSP-1, B-
333, E-Mail: a .
Vegetable stachys (Stachys sieboldii Mig.) is the tuber-forming perennial
plant from Labiatae family. It has valuable dietic properties because his
tubers possess o f some biologically active substances such as rare
tetrasaccharide stachiose that can substitute saccharose. Stachys is
propagated only by vegetative manner ( by tubers), so it is very interesting
to use cell culture methods to produce new forms o f plants and cell lines
with high productivity. So the first goal was to study the influence of
phytohormones to the callus and organ production by stachys explants. It
was found that 2,4-D was ineffective in callus induction at stachys: addition
of this auxin to culture medium causes necrotic processes and destruction
of ail explants (nodal and stem segments, leaf and root explants). With
NAA addition most explants had some soft callus with many roots. The
most effective in soft callus induction were 2,4,5-trichlorophenoxyacetic and
4-chlorophenoxyacetic acids. But the attempts to recultivate such callus
cause necrosis and its death even on the culture media with antioxidants.
Adventitious bud formation we observed at culture media with 1-2 mg/l
kinetin or 6-BAP only at the nodal explants having meristematic zones.
Sporadic shoot formation took place at the primary leaf callus. Spontaneous
rhizogenesis was observed at stem nodal segments after 3-7 days at the
hormone-free media. The addition of 0,1-1 mg/l IBA or NAA stimulated
rhizogenesis.
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