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П Л ЕН А РНЫ Е Д О К Л А ДЫ
гликозидов. Установлен ряд специфичных особенностей популяции
растительных клеток in vitro, например, неустойчивость популяции при
максимальных уровнях пролиферации. Показано, что варьируя режимы
культивирования можно эффективно управлять свойствами популяции:
изменять ее гетерогенность, жизнеспособность, интенсивность
пролиферации. Через управление популяционными характеристиками
возможно целенаправленно воздействовать на интенсивность вторичного
метаболизма.
Работа выполнена при поддержке Российского фонда фундаментальных
исследований (код проекта 96-04-49234).
HIGH PLANTPECUUARtTIESWHEN CONTINUOUS-FLOWCULTIVATING
Nosov A.M.
Timiriazev Plant Physiology Institute, 35 Botanical street, Moscow 127276, Russia, tel.
(095) 482-57-64,
High plant cell cultures can be considered as the experimentally produced
somatic cell populations. A priori, it can be supposed that a number of
these population peculiarities are resulted from the lack of prolonged evolution
influence. They are: the high lability, the environmental responsiveness and
the low stability. Thus, indicated peculiarities exhibite such plant cell population
as a unique model system fo r studying natural population initiation,
differentiation and perdiction.
On the other hand, its uniqueness is in the co-existence o f high plant
cells independent on the organismal control. Then, the main properties of
cultivating cells are: dedifferentness; the selection by maximum and/or stable
proliferation; a great amount of undemanded genetic information ( very
important at the organismic stage) and the possible preservation o f such
organism ic trait as secondary metabolism. These plant cell population
peculiarities and the significance o f above mentioned factors could be
thoroughly investigated by the methods of dosed and open continuous-flow
cultivation. The open continuous-flow cultivation ensures the convinient
conditions fo r cell auto-selection by "maximum proliferation extent” .
The closed continuous-flow cultivation is turned out to be purposely
modeling population aging under minimal growth limitation and inhibition.
These approaches have been used to research the cell population of
Dioscorea deltoidea Wail.( a producer of furostanol glycosides). It was found
out a number o f specific peculiariti es in in vitro plant cell population such
as its instability unde rthe maximum proliferation levels and etc.. Also, we
proved that varying the regimes of cultivation produced a possibility to control
efficiently some population properties such as its heterogeneity, viability
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