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Молочнохозяйственный вестник, №1 (29), I кв. 2018 82
БИОЛОГИЧЕСКИЕ И СЕЛЬСКОХОЗЯЙСТВЕННЫЕ НАУКИ
Influence of the polymorphism complex of κ-casein
(CSN3) and prolactin (PRL) genes on the milk productiv-
ity of the holstein first calf heifers
Safina Natalia Yur’evna, Junior Researcher; Postgraduate Student at Department
of Animal Technology
e-mail:
Federal State Budgetary Scientific Institution the Tatar Research Institute of
Agriculture, Kazan
Federal State Budgetary Educational Institution of Higher Education the Kazan
State Academy of Veterinary Medicine. N.E Bauman , Kazan
Yulmeteva Yuliana Rustemovna, PhD (Bio), Head of the Laboratory of Molecular
Genetic Studies;
e-mail:
Federal State Budgetary Scientific Institution the Tatar Research Institute of
Agriculture, Kazan
Shakirov Shamil Kasimovich, DSc (Agr), Professor, Head of the Scientific and
Technological Center of Animal Husbandry.
e-mail:
Federal State Budgetary Scientific Institution the Tatar Research Institute of
Agriculture, Kazan
Abstract. The presented study is dedicated to the influence of the polymorphism
complex of κ-casein (CSN3) and prolactin (PRL) genes, which are responsible for milk
quality and milk productivity. The study was carried out among Holstein first calf heifers
by Integrated Agricultural Production Centre “Breeding farm named after Lenin” of
Atninsky district of the Republic of Tatarstan. DNA samples were separated from blood
samples of 261 cows for identification and genotyping according to kappa-casein and
prolactin genes by PCR-RFLP analysis method. When considering the gene polymorphism
as whole cow-heifers with a complex CSN3AA – PRLAB genotype showed prominent results
on milk yield (6648 kg), with a CSN3AA – PRLAA genotype – on percent fat content
(3.84%), with a CSN3AB – PRLBB genotype – on percent protein (3.51%), with a CSN3BB
– PRLAA genotype – on milk fat (265.2 kg), with a CSN3AA – PRLAB genotype - on milk
protein content (210.6 kg). The study didn’t detect PRLBB – CSN3BB genotype complex.
Keywords: gen, PRL, CSN3, complex, polymorphism, PCR-RLFP, cattle, yield, fat,
protein, productivity Электронная Научная СельскоХозяйственная Библиотека