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Работа выполнена при финансовой поддержке межгосударственной целевой программы

ЕврАзЭС «Инновационные биотехнологии» №14.М04.12.0008.

Labelling of

Ppd

and

Vrn

genes in barley by means of PCR-analysis

Alabushev A.V.,

correspondent member of RAS,

Dontsova A.A.,

PhD in agriculture,

Potokina E.K.,*

DSc in biology

All-Russian Scientific-research institute of Grain Crops after I.G. Kalinenko,

Zernograd, Rostov region, Russia

*All-Russian N.I. Vavilov Institute of Plant Industry, St. Petersburg, Russia

During the last 15 years in Russia is observed the tendency of an increase in the average-daily tem-

perature (+ 1-2



С) toward the average perennial value of index, increase in the arid days and decrease of

atmospheric precipitations in the spring-summer period. One of the methods of increasing the yield of the

crop of barley is the creation of precocious varieties whose pouring in of grain passes to more comforta-

ble conditions in comparison with middle- and the late-maturing varieties. The involvement in the cross-

ings of the donors of early maturity will make it possible to conduct breeding in this direction more goal-

directed. For a barley molecular markers are worked out allowing to identify alleles of genes of photoper-

iodic reaction

Ppd

(photoperiod response) and reactions on the vernalization

Vrn

(vernalization response).

The genes

Ppd

determine the reaction of plants of barley on length of day and, as a result, terms of efflo-

rescing and beginning of earing of plants in the different growth conditions. The genes

Vrn

determine

requirement of plants in a vernalization for passing to earing; thus participated in regulation of speed of

development and expressed of yield structure in barley.

In this study with help of an allele-specific mo-

lecular markers it is reveal that an early flowering barley variety Master bred in All-Russian Research

Institute of Grain Crops carries the dominant

Ppd-H1

allele that might explain its fast heading on the long

photoperiod. It is recommended to use the Master variety as a donor of early maturing in breeding pro-

grams. Besides barley variety Master has winter-and-spring character which is testified by detection of

recessive allele of

vrn-H2

gene.

Key words:

winter-and-spring barley, drought, precocity, PСR analysis, Ppd-H1, Vrn-H2, molecu-

lar markers, donor

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