ЭлБиб

Mediterranean Agricultural Research Institute were screened for resistance to charcoal rot in the

field under natural infection conditions and in the greenhouse with artificial inoculation in Diyarbakir.

Pot tests.

For inoculum, an agar plug from an actively growing M

phaseofitui

culture was

placed in a 500-ml flasks containing sterile boiled oat kernel. Flasks were incubated at 32-34°C for

15 days (Mihail, 1992). Inoculum was added directly to podding soil at the rate o f 40g/pot. Potting

soil was watered during ten days. On the other hand, sesame seeds were surface-sterilized in a

!%NaOCL solution for 1-2 min and air-dried on filter paper in the shade. Then, disinfested seeds

were sown in I5cm-diameter pots containing infested soil and manure ( l/l, v/v). The experiment

was arranged in a randomized complete block design with four replications. Inoculated plants were

grown under controlled greenhouse conditions.

Field tests.

Field experiments were conducted in soils naturally infested with

M. phaseolina

Plant Protection, Research Institute, Diyarbakir, Turkey, during

1998

and

1999.

Field plots were

established in a randomized complete block design with four replications. To increase inoculum in

field soil, oat-kernel inoculum was incorporated into soil at the rate o f 300g/plot. Recommended

crop management practices were followed throughout the season

In the evaluation done based on disease incidence, plants were rated on a scale o f 0-4 (0;

resistant, no infection, ,1; less susceptible, 0-25%diseased plant, 2;..intermediate resistant, 25-

50%diseascd plant, 3; highly susceptible, <50% diseased plant, 4, died plant). All plants were

evaluated according to results o f both tests, Data were analysed by analyses of variance (ANOVAs)

with MINITAB . Means were compared by Duncan’s Multiple Range Test (IM),05).

RESULTS AND DISCUSSION

In evaluations o f the resistance o f sesame to

M. pltaseolina,

line/cultivars from Izmir,

Menemen Plant Genetics Resources Research Institute, Mediterranean and Aegean Agricultural

Research Institutes were grown in 1998 and ,1999 in the field natural infection conditions and in the

greenhouse with artificial inoculation. Experiments were carried out during 1998 and 1999 at both

seedling and flowering-pod stages. The fungus was originally isolated from diseased sesame plants

taken from the sesame growing areas o f the Southeastern Anatolia Region Both pot and field tests

showed that o f the 147 linc/cultivars tested, 23 (Ant-59, Tur-5-151, Tur-S-179, Tur-S-123, Tur-S-

51, Tur-S-190,. Muganli-57, Tur-S-176, Tur-S-191, Ant-57, Tur-S-155, Tur-S-146, Golmarmara,

Tur-S-161, Tur-S-172, 29/9-4-2, Tur-S-131, Aksu, Tur-S-101; Tur-S-147, Tur-S-17i; Camdibi and

31594) were found to be resistant to charcoal rot. The lowest incidence o f the disease was observed

on these line/cultivars at the rate o f 0.0-15.0%. 35 introduced line/cultivars were moderately

resistant. These cultivate had infection levels of 49-70%. The rest (89 line/cultivars) were rejected as :

susceptible or highly susceptible with disease incidence at the rate o f 71-100% Some o f them died

due to pith maceration There was a lot of black sclerotia like charcoal all over the dried plants.

These result were agree with findings of Ata$ et a! (1995) studying on root on crown rot disease o f

sesame and those o f Amar et al. (1989) working on reaction of sesamum germplasm/cultivars against

Macropltomina

root rot.

ACKNOWLEDGEMENTS

•(

This research was supported by “ The Scientific and Technical Research Council of Turkey

(TUB1TAK )”, Project no. TOGTAG-I ARP- 1916.

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