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superoxide. Present work suggests that lack of catalase activity in the
katG
mutant of
Synechocystis
sp. PCC 6803 does not sensitize to MV
because Fe-SOD is active. However, if the Fe-SOD is absent, as in the
Synechococcus
sp. PCC 7942
sodB
mutant, activation of catalase
appears to be an adaptive response to MV stress that may allow
survival, if not continued growth.
Table 2. Catalase activity in the
Synechococcus
sp. PCC 7942 wild
type and
sodB'
mutant cultures started at normal growth conditions and
then exposed to 0.5 p.M MV at the same growth conditions. Activity
was measured in wю!е cells suspensions according to [7]
Synechococcus
sp. PCC 7942
Time of
growth, hr
Catalase activity, цто! min'110‘6
cells (means ± SE)
Control
(without MV)
With 0.5 pM
MV
Wild type
4
12.40 ±0.66
14.39 ±0.78
6
12.68 ±0.67
11.15 ± 0.15
30
12.35 ±0.15
16.91 ±0.22
50
11.56 ±0.59
11.39 ± 0.63
SodB'
mutant
4
13.54 ±0.35
35.63 ± 0.43
6
12.85 ± 0.36
37.89 ± 0.76
30
11.89 ±0.22
36.42 ± 0.46
50
15.53 ±0.22
38.30 ± 0.46
This work was supported by grant from the U.S. Civilian
Research and Development Foundation; project # RB1-2351-PU-02.
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