1-248.indd

61 Болезни сельскохозяйственных животных UDC 619:616.98:578.833.31:616-078 MAIN CHARACTERISTICS OF INDIRECT E2-ELISA DEVELOPED FOR DETECTION OF ANTIBODIES TO CLASSICAL SWINE FEVER VIRUS A.V. Kanshina 1 , А.S. Yakovleva 2 , Ye.S. Orlova 3 , А.V. Scherbakov 4 1 Senior Researcher, Candidate of Science (Veterinary Medicine), FGBI «ARRIAH», Vladimir; е-mail: kanshina@arriah.ru 2 Senior Researcher, Candidate of Science (Biology), FGBI «ARRIAH», Vladimir; е-mail: yakovleva_as@arriah.ru 3 Senior Researcher, Candidate of Science (Biology), FGBI «ARRIAH», Vladimir; е-mail: orlova@arriah.ru 4 Head of Laboratory, Candidate of Science (Biology), FGBI «ARRIAH», Vladimir; е-mail: ascherbakov@arriah.ru Summary Main characteristics of indirect variant of enzyme-linked immunosor- bent assay with recombinant E2 protein as an antigen (E2-ELISA) for the detection of antibodies against classical swine fever virus are given in the paper. E2-ELISA is found to be highly stable and precise as for its repeatability and reproducibility. The assay diagnostic specificity was 99,78%, and diagnostic sensitivity was 97,18%. Routine diagnostic tests confirmed high specificity and sensitivity of the assay determined during its development. Key words: validation, classical swine fever, antibodies, enzyme- linked immunosorbent assay. Электронная Научная СельскоХозяйственная Библиотека