1-248.indd

176 Биотехнология UDС 619:578.835.2:616-076 USE OF AMPICILLIN, CIPROFLOXACIN, CEFOTAXIME AND POLYMYXIN B FOR PREVENTION OF BHK-21/2-17 CELL LINE MICROBIAL CONTAMINATION M.I. Doronin 1 , A.A. Shishkova 2 , N.D. Klyukina 3 , D.A. Lozovoy 4 , D.V. Mikhalishin 5 1 Junior Researcher, Candidate of Science (Biology), FGBI «ARRIAH», Vladimir; e-mail: doronin@arriah.ru 2 Chief Technologist, Candidate of Science (Veterinary Medicine), FGBI «ARRIAH», Vladimir; e-mail: shishkova@arriah.ru 3 Senior Researcher, Candidate of Science (Veterinary Medicine), FGBI «ARRIAH», Vladimir; e-mail: klukina@arriah.ru 4 Director, Candidate of Science (Veterinary Medicine), FGBI «ARRIAH», Vladimir; e-mail: lozovoy@arriah.ru 5 Head of Laboratory, Candidate of Science (Veterinary Medicine), FGBI «ARRIAH», Vladimir; e-mail: michalishin_dv@arriah.ru Summary The paper describes the use of ampicillin, ciprofloxacin, cefotaxime and polymyxin B for the prevention of bacterial and mycoplasma con- tamination in suspension continuous BHK-21/2-17 cell culture. Optimal antibiotic concentrations were determined for ampicillin (1,500 μg/ml or 2,400 U/ml), ciprofloxacin (17 μg/ml), cefotaxime (60 μg/ml or 55 U/ml), polymyxin B (13 μg/ml or 100 U/ml), which are used for cultivation of this cell line. The use of antibiotics in the proposed doses prevented the growth of microorganisms in cells without causing mor- phological changes or reducing proliferative capacity of BHK-21/2-17 cell line. Key words : BHK-21/2-17 cell line, proliferative activity index, ampi- cillin, ciprofloxacin, cefotaxime, polymyxin B, optimal antibiotic dose, microbial contamination. Электронная Научная СельскоХозяйственная Библиотека