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132 Болезни птиц UDC 619:616.98:578.831.3:636.5:616-073 STUDYING IMMUNE RESPONSE IN BROILER CHICKENS TO AVIAN METAPNEUMOVIRUS SUBTYPE A P.S. Yaroslavtseva 1 , M.A. Volkova 2 , V.Yu. Sosipatorova 3 , T.I. Yeroshina 4 1 Junior Researcher, FGBI «ARRIAH», Vladimir; e-mail: yaroslavtseva@arriah.ru 2 Leading Researcher, Candidate of Science (Biology), FGBI «ARRIAH», Vladimir; e-mail: volkovama@arriah.ru 3 Biologist, Postgraduate Student, FGBI «ARRIAH», Vladimir; e-mail: sosipatorova@arriah.ru 4 Leading Technologist, FGBI «ARRIAH», Vladimir; e-mail: mail@arriah.ru Summary Seven-day old broiler chickens were experimentally infected with avi- an metapneumovirus subtype A. On Days 6-14 post infection the follow- ing clinical signs were observed: respiratory infection, swollen infraor- bital sinuses. Pathological lesions were detected in the upper respiratory tract. Real-time RT-PCR was used to detect avian avian metapneumovirus genome in nasal cavity tissues and in lungs of infected chickens on Days 1, 3 and 7 post infection. Infection of chickens with avian metapneumo- virus resulted in local immune responses expressed as development of specific antibodies (three classes A, M, G) in lachrymal and oropharyn- geal fluid on days 7-28 post infection. Specific antibodies in sera samples were detected only in 20% of infected chickens. Infection of chickens with avian avian metapneumovirus did not cause an increase in cytotoxic activity of T-cells and in spleen production of concanavalin A-induced interferon - gamma. Key words: avian metapnumovirus, metapneumovirus infection, cell and humoral immunity, pathogenesis. Электронная Научная СельскоХозяйственная Библиотека