NED373393NED

70 Болезни сельскохозяйственных животных RESEARCH STUDY OF SUBGROUP J AVIAN LEUCOSIS VIRUS SPREAD IN THE TERRITORY OF THE RUSSIAN FEDERATION M.I. Shulpin 1 , A.E. Menschikova 2 , S.P. Lazareva 3 1 leading research worker, PhD (Biology), FGBI «ARRIAH», Vladimir; E-mail: shulpin@arriah.ru 2 senior research worker, PhD (Biology), FGBI «ARRIAH», Vladimir 3 leading biologist, FGBI «ARRIAH», Vladimir Summary It was established as a result of monitoring in 2008 – 2012 that avian leucosis virus ALV-J was widely spread on poultry farms of the Russian Federation. Farms with cases of virus persistence were identified using virological, serological and molecular-biological methods. Samples from 14 poultry farms were positive based on results of the polymerase chain reaction; it constituted 25% out of the total number of poultry farms under examination. ALV-J antibodies were detected in chicken blood sera from 70% of farms both egg-type and broiler-type which sent samples to the FGBI «ARRIAH» for testing. Key words: avian leucosis virus, ELISA, solid-phase immunosorbent assay, polymerase chain reaction, chicken fibroblast culture. Электронная Научная СельскоХозяйственная Библиотека