NED373392NED

Болезни сельскохозяйственных животных 145 CULTIVATION OF FIELD ISOLATES OF AVIAN LEUKOSIS VIRUS SUBGROUP J RECOVERED IN THE TERRITORY OF THE RUSSIAN FEDERATION IN 2008–2010 S.I. Lazareva 1 , A.N. Ushakova 2 1 leading biologist, FGBI «ARRIAH», Vladimir; Е-mail: lazareva@arriah.ru 2 leading biologist, FGBI «ARRIAH», Vladimir Summary Six isolates of avian leukosis virus subgroup J (ALV-J) were recovered from pathological materials submitted from various poultry farms. Chick embryo fibroblast (CEF) culture was used as a cultivation system. Yielded virus-containing material was tested using real-time polymerase chain reac- tion (PCR) and enzyme-linked immunosorbent assay (ELISA). Ct values determined by PCR following final passages varied from 13 to 27. ALV-J VLD-1005 sample had maximal real-time PCR Ct value (Ct 13). Concentra- tion of p27 viral protein determined using ELISA was 10 3 –10 4 pkg/cm 3 . ALV-J KOM1010 isolate-containing sample was found to have maximal viral protein concentration (4,3×10 4 pkg/cm 3 ). Key words : avian leukosis virus subgroup J, chick embryo fibroblast culture, real-time polymerase chain reaction, enzyme-linked immunosorbent assay Электронная Научная СельскоХозяйственная Библиотека