NED373390NED

Ящур и другие болезни крупного рогатого скота и свиней 81 науч.-произв. конф., посвящ. 100-летию со дня рождения члена-корреспондента ВАСХНИЛ В.Т. Котова. – Воронеж, 1999. – С. 93-95. 5. Инфекционная патология животных: в 2 т. Т.2. / под ред. А.Я. Самуйленко, Б.В. Соловьева, Е.А. Непоклонова, Е.С. Воронина.– М.: Академкнига, 2006. – 807 с. 6. Jakobson, R.H. Validation of serological assays for diagnosis of infectious diseases // Rev.Sci. Techn. OIE. – 1998. – Vol. 17, N 2. – P. 469-486. 7. Phylogenetic analysis of porcine rotavirus in Argentina: Increasing diversity of G4 strains and evidence of interspecies transmission / G.I. Parra, G. Vidales, J.A. Gomez [et al.] // Vet. Microbiology. – 2008. – Vol. 126. – P. 243-250. 8. Rapid serological profiling by enzyme-linked immunosorbent assay. 2. Comparison of computation methods for measuring antibody titers in a single serum dilution / D.B. Snyder, W.W. Marquardt, E.T. Mallison [et al.] // Avian Dis. – 1982. – Vol. 27. – P. 474-484. DEVELOPMENT of ELISA test-system for detection of antibodies to porcine retrovirus using a single dilution method A.S. Oganesyan, O.P. Byadovskaya, O.G. Andreyeva, L.B. Prokhvatilova Summary An ELISA test-system for detection of antibodies to rotavirus in porcine sera was developed. Cultural inactivated purified concentrated porcine rotavirus KR-47 strain was used as antigen. Results of comparative testings of porcine sera with the developed ELISA and Ingezim rotavirus ELISA test-kit (INGENAZA, Spain) are given. The relative specificity and sensitivity of the developed method were determined. The given ELISA test-system was used for determination of a level of rotavirus spread in pigs on pig farms of the Central Federal Okrug of the Russian Federation (CFO RF). Электронная Научная СельскоХозяйственная Библиотека